Electron Microscopy for the Analysis of Peripheral Nerve Myelin

Methods Mol Biol. 2018:1791:3-13. doi: 10.1007/978-1-4939-7862-5_1.

Abstract

Transmission electron microscopy (TEM) can provide excellent high-resolution images of cellular structures. A critical step with this technique is sample preparation. Here we describe our protocol for the preparation of semithin and ultrathin sections of mouse peripheral nerves in order to visualize the structure and pathological features of peripheral myelin with TEM.

Keywords: Myelin; Periodicity; Sample preparation; Schwann cell; Semithin sectioning; TEM; Ultrathin sectioning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Mice
  • Microscopy, Electron* / methods
  • Microscopy, Electron, Transmission / methods
  • Myelin Sheath / ultrastructure*
  • Peripheral Nerves / ultrastructure*
  • Schwann Cells / metabolism
  • Schwann Cells / ultrastructure