Myelin Protein Zero180-199 Peptide Induced Experimental Autoimmune Neuritis in C57BL/6 Mice

Methods Mol Biol. 2018:1791:243-250. doi: 10.1007/978-1-4939-7862-5_19.

Abstract

Mouse models of peripheral demyelinating neuropathy play an important role in enabling the study of disease pathogenesis. Further, induction in transgenic mice allows for the precise interrogation of disease mechanisms, as well as the analysis of the efficacy and mechanisms of potential new therapies. Here we describe a method to successfully induce experimental autoimmune neuritis (EAN) using myelin protein zero (P0)180-199 peptide in combination with Freund's complete adjuvant and pertussis toxin in the C57BL/6 mouse strain. We also outline a sensitive paradigm of accurately assessing the extent of functional deficits occurring in murine EAN.

Keywords: C57BL/6 mice; Experimental autoimmune neuritis; Myelin; P0; Peripheral neuropathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Disease Models, Animal
  • Gait
  • Mice
  • Mice, Inbred C57BL
  • Motor Activity
  • Myelin P0 Protein / chemistry
  • Myelin P0 Protein / immunology*
  • Myelin P0 Protein / metabolism
  • Neuritis, Autoimmune, Experimental / diagnosis
  • Neuritis, Autoimmune, Experimental / immunology*
  • Neuritis, Autoimmune, Experimental / metabolism
  • Neuritis, Autoimmune, Experimental / physiopathology
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology*
  • Peptide Fragments / metabolism
  • Pertussis Toxin / administration & dosage
  • Pertussis Toxin / adverse effects
  • Pertussis Toxin / immunology

Substances

  • Myelin P0 Protein
  • Peptide Fragments
  • Pertussis Toxin