Extrachromosomal circular DNA and aging cells

Adv Exp Med Biol. 1985:190:479-93. doi: 10.1007/978-1-4684-7853-2_24.

Abstract

A DNA sequence situated in the human genome between Alu-repeat clusters ("Inter-Alu" DNA) is progressively amplified in extrachromosomal DNA, including covalently closed DNA circles, during serial passage of diploid fibroblasts. A single size-class of Inter-Alu circles is also amplified in lymphocytes from 16 of 24 old donors and yet is not detected in cells from 18 young donors.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Survival*
  • Chromosomes, Human
  • DNA Transposable Elements
  • DNA, Circular / analysis*
  • Fibroblasts / chemistry
  • Gene Amplification
  • Humans
  • In Vitro Techniques
  • Lymphocytes / chemistry
  • Repetitive Sequences, Nucleic Acid

Substances

  • DNA Transposable Elements
  • DNA, Circular