3' c-myc rearrangement in a human leukemic T-cell line

Cancer Res. 1986 Mar;46(3):1413-7.

Abstract

The human leukemic T-cell line Hut 78, derived from a patient suffering from Sézary syndrome and expressing a mature postthymic membrane phenotype, shows a c-myc rearrangement beginning within 500 base pairs immediately 3' to the c-myc exon 3. Chromosome analysis of the Hut 78 reveals the presence of a hyperdiploid karyotype with a large number of markers and rearrangements, though trisomy is the only cytogenetic anomaly involving chromosome 8. Moreover, as the abnormal c-myc appears to be duplicated, a duplication of the chromosome 8 carrying the abnormal c-myc probably occurred. Unlike four other human leukemic T-cell lines tested, the Hut 78 cells express a high amount of c-myc transcript, suggesting that the 3' c-myc anomaly may cause a deregulation of the expression of this gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • DNA Restriction Enzymes
  • DNA, Neoplasm / genetics*
  • Gene Expression Regulation
  • Genes
  • Humans
  • Karyotyping
  • Proto-Oncogenes*
  • Sezary Syndrome / genetics*
  • T-Lymphocytes / physiology*

Substances

  • DNA, Neoplasm
  • DNA Restriction Enzymes