The microemulsion electrokinetic capillary chromatography combined with reversed-electrode polarity stacking mode for enriching and quantifying lignanoids and ginsenosides in TCMs preparation Shengmai injection

Electrophoresis. 2018 Oct;39(19):2439-2445. doi: 10.1002/elps.201800196. Epub 2018 Jul 29.

Abstract

An on-line large volume sample stacking with polarity switching (LVSS) method was proposed for simultaneously determining lignanoids and ginsenosides in MEEKC. The parameters including the pH value and concentration of buffer solution, SDS, organic modifier, oil phase, running voltage, and temperature as well as injection time, sample matrix, stacking voltage, and time influencing separation and stacking were systematically optimized. The method was verified by performing precision, accuracy, stability, and recovery. Its reliability was proved by separating and quantifying two lignanoids and three ginsenosides in Shengmai injectionSMI. The sensitivity of these compounds was improved by MEEKC-LVSS method for 6-11 times than conventional MEEKC. Thus, this developed on-line MEEKC-LVSS method was sensitive, practical, and reliable.

Keywords: LVSS; Lignanoids and ginsenosides; Microemulsion electrokinetic chromatography; Shengmai injection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Micellar Electrokinetic Capillary / methods*
  • Emulsions / chemistry
  • Ginsenosides / analysis*
  • Ginsenosides / chemistry
  • Ginsenosides / isolation & purification
  • Lignans / analysis*
  • Lignans / chemistry
  • Lignans / isolation & purification
  • Limit of Detection
  • Linear Models
  • Reproducibility of Results

Substances

  • Emulsions
  • Ginsenosides
  • Lignans