De novo assembly and comparative transcriptome analysis reveals genes potentially involved in tissue-color changes in centipedegrass (Eremochloa ophiuroides [Munro] Hack.)

Turf color is the most important characteristics of visual quality for a turfgrass species with high ornamental value and wide application prospects. Centipedegrass is a well-adapted warm-season turfgrass species in tropical, subtropical and temperate regions, possessing many outstanding properties including uniform green color. However, quite a few centipedegrass accessions or cultivars produce stolons and spike tissues with red-purple color, thereby decreasing their aesthetic value. A research focus in centipedegrass is to develop high-quality cultivars with uniform green color. To explore the major genes associated with the color changes in certain organs/tissues contributes to understand the molecular mechanisms of the same tissues having different phenotypic characteristics. In the present study, two phenotypically distinct centipedegrass accessions, E092 being a wild-type (WT) with red-purple stolons and spike tissues and E092-1 being a mutant (MT) with uniform green stolons and spike tissues, were used. Using the Illumina sequencing platform, approximately 401.7 million high-quality paired-end reads were obtained. After de novo assembly and quantitative assessment, 352,513 transcript sequences corresponding to 293,033 unigenes were generated with an average length of 735 bp. A total of 145,032 (49.49%) unigenes were annotated by alignment with public protein databases. Of these unigenes, 329 differentially expressed genes (DEGs) were identified between WT and MT stolons, with 156 up-regulated and 173 down-regulated; and 829 DEGs were detected between WT and MT spike tissues, including 497 up-regulated and 332 down-regulated. The expression profile of 10 randomly selected DEGs was confirmed with RT-qPCR. Candidate genes involved in the flavonoid biosynthesis were identified showing significant transcript changes between WT and MT organs/tissues. And transcript abundances of these flavonoid biosynthetic pathway-related genes were positively correlated with the accumulation of total anthocyanin in respective organs/tissues. This assembled transcriptome of centipedegrass can be served as a global description of expressed genes of above-ground organs/tissues and provide more molecular resources for future functional characterization analysis of genomics in warm-season turfgrass. Identified genes related to centipedegrass organ/tissue changes will contribute to molecular improvement of turf quality through genetic manipulation.