Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis

Clin Immunol. 2018 Dec:197:19-26. doi: 10.1016/j.clim.2018.07.014. Epub 2018 Jul 26.

Abstract

B-cell activating factor (BAFF) has been proposed to play a crucial role in the pathogenesis of chronic rhinosinusitis with nasal polyp (CRSwNP). The aim of this study was to evaluate the role of toll-like receptor (TLR) 9-mediated BAFF activation on the pathogenesis of CRSwNP. NP and uncinate tissue (UT) were obtained from patients with CRSwNP or CRS without NP, and control subjects. The expression of TLR9, high mobility group box-1 protein (HMGB1), type I interferon (IFN), BAFF, and anti-double stranded DNA (dsDNA) antibody were examined in the tissues and the cultured dispersed NP cells (DNPCs). The expression of TLR9, HMGB1, type I IFN, BAFF, and anti-dsDNA antibody were elevated in NP tissue compared to the UTs. Exposure to TLR9 agonist increased the type I IFN expression in vitro, which further increased BAFF production. In conclusion, we provided a novel therapeutic potential of TLR9 agonist in CRSwNP.

Keywords: B-cell activating factor; Nasal polyp; Sinusitis; Toll-like receptor; Type I interferon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • B-Cell Activating Factor / drug effects
  • B-Cell Activating Factor / genetics*
  • B-Cell Activating Factor / metabolism
  • Chronic Disease
  • Female
  • Frontal Sinus / metabolism
  • HMGB1 Protein / genetics*
  • HMGB1 Protein / metabolism
  • Humans
  • In Vitro Techniques
  • Interferon-alpha / drug effects
  • Interferon-alpha / genetics
  • Interferon-alpha / metabolism
  • Interferon-beta / drug effects
  • Interferon-beta / genetics
  • Interferon-beta / metabolism
  • Male
  • Middle Aged
  • Nasal Polyps / metabolism*
  • RNA, Messenger / metabolism
  • Rhinitis / metabolism*
  • Sinusitis / metabolism*
  • Toll-Like Receptor 9 / agonists
  • Toll-Like Receptor 9 / metabolism*

Substances

  • B-Cell Activating Factor
  • HMGB1 Protein
  • HMGB1 protein, human
  • Interferon-alpha
  • RNA, Messenger
  • TLR9 protein, human
  • TNFSF13B protein, human
  • Toll-Like Receptor 9
  • Interferon-beta