Time Matters: Gene Editing at the Mouse 2-Cell Embryo Stage Boosts Knockin Efficiency

Alvaro Plaza Reyes; Fredrik Lanner

doi:10.1016/j.stem.2018.07.008

Time Matters: Gene Editing at the Mouse 2-Cell Embryo Stage Boosts Knockin Efficiency

Cell Stem Cell. 2018 Aug 2;23(2):155-157. doi: 10.1016/j.stem.2018.07.008.

Authors

Alvaro Plaza Reyes¹, Fredrik Lanner²

Affiliations

¹ Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 14186 Stockholm, Sweden; Ming Wai Lau Center for Reparative Medicine, Karolinska Institutet, 14186 Stockholm, Sweden; Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset, 14186 Stockholm, Sweden.
² Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 14186 Stockholm, Sweden; Ming Wai Lau Center for Reparative Medicine, Karolinska Institutet, 14186 Stockholm, Sweden; Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset, 14186 Stockholm, Sweden. Electronic address: [email protected].

PMID: 30075125
DOI: 10.1016/j.stem.2018.07.008

Abstract

Although endonuclease-mediated genome editing techniques offer significant improvement over traditional methods, they are still ineffective for introduction of large DNA sequences. Recently in Nature Biotechnology, Gu et al. (2018) developed a CRISPR-Cas strategy termed 2C-HR-CRISPR that generates fluorescent reporter tagging of genes with up to 95% knockin efficiency in mouse embryos.

Publication types

Comment

MeSH terms

Animals
CRISPR-Cas Systems*
Gene Editing*
Mice
Microinjections