Calcium (Ca2+ ) signaling controls T-cell activation and functions. Ca2+ concentrations are locally detected and controlled by Ca2+ -sensors (STIM1 and 2 detecting the depletion from ER stores channels) and Ca2+ -channels (ORAI1-3 in the cell membrane and VDAC1 in the outer mitochondrial membrane). We first validated and titrated antibodies to assess the expression of these Ca2+ -sensors and -channels in human and murine cells, and further devised a 18-antibodies mass cytometry panel to characterize their expression in primary murine lymphocyte subsets.
Keywords: calcium release-activated calcium channels (CRAC/ORAI); mass cytometry; murine primary lymphocyte subsets; panel design; stromal interaction molecule (STIM); voltage-dependent anion-selective channel protein (VDAC).
© 2018 International Society for Advancement of Cytometry.