Pyrazinium thioacetate capped gold nanoparticles as Fe(III) sensor and Fe(III) marked anti-proliferating agent in human neuroblastoma cells

Ayaz Anwar; Aaliya Minhaz; Syed Saad Hussain; Areeba Anwar; Shabana Usman Simjee; Muhammad Ishaq; Naveed Ahmed Khan; Muhammad Raza Shah

doi:10.1016/j.saa.2018.07.099

Pyrazinium thioacetate capped gold nanoparticles as Fe(III) sensor and Fe(III) marked anti-proliferating agent in human neuroblastoma cells

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 5:206:135-140. doi: 10.1016/j.saa.2018.07.099. Epub 2018 Aug 1.

Authors

Ayaz Anwar¹, Aaliya Minhaz², Syed Saad Hussain³, Areeba Anwar⁴, Shabana Usman Simjee³, Muhammad Ishaq⁵, Naveed Ahmed Khan⁶, Muhammad Raza Shah⁷

Affiliations

¹ Department of Biological Sciences, School of Science and Technology, Sunway University, Subang Jaya 47500, Selangor, Malaysia; H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. Electronic address: [email protected].
² Department of Chemistry, Shaheed Benazir Bhutto Women University Peshawar, Peshawar 25120, Khyber Pakhtoon Khwa, Pakistan.
³ H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
⁴ Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
⁵ Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Khyber Pakhtoon Khwa, Pakistan.
⁶ Department of Biological Sciences, School of Science and Technology, Sunway University, Subang Jaya 47500, Selangor, Malaysia.
⁷ H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. Electronic address: [email protected].

PMID: 30096697
DOI: 10.1016/j.saa.2018.07.099

Abstract

Gold nanoparticles (AuNPs) stabilized by new cationic 1‑(3‑(acetylthio)propyl)pyrazin‑1‑ium ligand (PPTA) were synthesized. AuNPs stabilized by PPTA (PPTA-AuNPs) were found to be spherical and polydispersed with the average size of 60 nm. Human neuroblastoma (SHSY-5Y) cells permeability of PPTA-AuNPs was found to be a key feature to study the intracellular quenching of Fe(III) proliferative activity. In vitro MTT assay revealed non-cytotoxicity of PPTA and PPTA-AuNPs at 100 μM concentration, while treatment of 100 μM of Fe(III) with SHSY-5Y cells resulted into higher cells viability. Contrary, a mixture of 1:1 Fe(III) with PPTA-AuNPs showed no change in the viability of cells at same concentration which suggests the intracellular complexation and recognition of Fe(III) by PPTA-AuNPs. AFM morphological analysis of SHSY-5Y cells also supported the MTT assay results, and it is safe to conclude that PPTA-AuNPs can be used as Fe(III) probes in living cells. In addition, Fe(III) caused a significant decrease in the absorbance of surface plasmon resonance (SPR) band of PPTA-AuNPs in a wide range of concentration and pH, with limit of detection 4.3 μM. Moreover, the specific response of PPTA-AuNPs towards Fe(III) was unaffected by the interference of other metals and components of real samples of tap water.

Keywords: AFM; Fe(III); Gold nanoparticles; Neuroblastoma cells; Pyrazine; Surface plasmon resonance.

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology
Cell Line, Tumor
Cell Proliferation / drug effects
Ferric Compounds / analysis*
Gold / chemistry
Humans
Limit of Detection
Metal Nanoparticles / chemistry
Neuroblastoma / metabolism*
Pyrazines / chemistry*
Sulfhydryl Compounds / chemistry*

Substances

Antineoplastic Agents
Ferric Compounds
Pyrazines
Sulfhydryl Compounds
Gold
thioacetic acid