Practical considerations for clinical use of mesenchymal stem cells: From the laboratory to the horse

Vet J. 2018 Aug:238:49-57. doi: 10.1016/j.tvjl.2018.07.004. Epub 2018 Jul 17.

Abstract

Since the clinical use of mesenchymal stem cells (MSCs) for treating musculoskeletal injuries is gaining popularity, practitioners should be aware of the factors that may affect MSCs from tissue harvesting for MSC isolation to cell delivery into the injury site. This review provides equine practitioners with up-to-date, practical knowledge for the treatment of equine patients using MSCs. A brief overview of laboratory procedures affecting MSCs is provided, but the main focus is on shipping conditions, routes of administration, injection methods, and which commonly used products can be combined with MSCs and which products should be avoided as they have deleterious effects on cells. There are still several knowledge gaps regarding MSC-based therapies in horses. Therefore, it is important to properly manage the factors which are currently known to affect MSCs, to further strengthen the evidence basis of this treatment.

Keywords: Horse; Mesenchymal stem cells; Practical considerations; Product combinations; Transport.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Horse Diseases / therapy*
  • Horses
  • Mesenchymal Stem Cell Transplantation / methods
  • Mesenchymal Stem Cell Transplantation / veterinary*
  • Mesenchymal Stem Cells / cytology*
  • Musculoskeletal Diseases / therapy
  • Musculoskeletal Diseases / veterinary*
  • Regenerative Medicine