Abstract
Re-domestication of potato into an inbred line-based diploid crop propagated by seed represents a promising alternative to traditional clonal propagation of tetraploid potato, but self-incompatibility has hindered the development of inbred lines. To address this problem, we created self-compatible diploid potatoes by knocking out the self-incompatibility gene S-RNase using the CRISPR-Cas9 system. This strategy opens new avenues for diploid potato breeding and will also be useful for studying other self-incompatible crops.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CRISPR-Associated Protein 9
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CRISPR-Cas Systems
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Diploidy*
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Gene Knockdown Techniques / methods*
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Genes, Plant / genetics
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Genes, Plant / physiology
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Phylogeny
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Plant Proteins / genetics*
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Plant Proteins / physiology
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Plants, Genetically Modified
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Pollination* / genetics
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Pollination* / physiology
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Polymerase Chain Reaction
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Ribonucleases / genetics*
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Ribonucleases / physiology
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Self-Fertilization* / genetics
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Self-Fertilization* / physiology
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Self-Incompatibility in Flowering Plants / genetics
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Solanum tuberosum / genetics*
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Solanum tuberosum / physiology
Substances
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Plant Proteins
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CRISPR-Associated Protein 9
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Ribonucleases