Discrepancies in fosfomycin susceptibility testing of KPC-producing Klebsiella pneumoniae with various commercial methods

Giulio Camarlinghi; Eva Maria Parisio; Alberto Antonelli; Maria Nardone; Marco Coppi; Tommaso Giani; Romano Mattei; Gian Maria Rossolini

doi:10.1016/j.diagmicrobio.2018.07.014

Discrepancies in fosfomycin susceptibility testing of KPC-producing Klebsiella pneumoniae with various commercial methods

Diagn Microbiol Infect Dis. 2019 Jan;93(1):74-76. doi: 10.1016/j.diagmicrobio.2018.07.014. Epub 2018 Jul 31.

Authors

Giulio Camarlinghi¹, Eva Maria Parisio¹, Alberto Antonelli², Maria Nardone¹, Marco Coppi², Tommaso Giani², Romano Mattei¹, Gian Maria Rossolini³

Affiliations

¹ Operative Unit of Chemical-Clinical and Microbiological Analysis; San Luca Hospital Usl Toscana Nord Ovest, Lucca, Italy.
² Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
³ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy. Electronic address: [email protected].

PMID: 30126624
DOI: 10.1016/j.diagmicrobio.2018.07.014

Abstract

Fosfomycin susceptibility testing with Sensititre, Vitek2, Etest, Mic Strip Test and disk diffusion methodologies was compared versus reference agar dilution method (AD) with 78 clinical isolates of KPC-producing Klebsiella pneumoniae. All methodologies showed a Categorical Agreement and Essential Agreement of ≤69% and ≤72%, respectively, revealing a very low concordance with AD.

Keywords: Agar dilution; CPE; CRE; Fosfomycin-resistance; KPC-KP.

Publication types

Comparative Study

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / analysis
Bacterial Proteins / metabolism*
Diagnostic Errors
Drug Resistance, Bacterial / drug effects
Fosfomycin / pharmacology*
Humans
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects*
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests / methods*
Microbial Sensitivity Tests / statistics & numerical data
Reagent Kits, Diagnostic / statistics & numerical data
beta-Lactamases / analysis
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Reagent Kits, Diagnostic
Fosfomycin
beta-Lactamases
carbapenemase