Dietary Galacto-Oligosaccharides and Resistant Starch Protect Against Altered CB1 and 5-HT1A and 2A Receptor Densities in Rat Brain: Implications for Preventing Cognitive and Appetite Dysfunction During a High-Fat Diet

Mol Nutr Food Res. 2018 Nov;62(21):e1800422. doi: 10.1002/mnfr.201800422. Epub 2018 Sep 9.

Abstract

Scope: A high-fat, but low-fiber, diet is associated with obesity and cognitive dysfunction, while dietary fiber supplementation can improve cognition.

Methods and results: This study examines whether dietary fibers, galacto-oligosaccharides (GOS) and resistant starch (RS), could prevent high-fat (HF)-diet-induced alterations in neurotransmitter receptor densities in brain regions associated with cognition and appetite. Rats are fed a HF diet, HF diet with GOS, HF diet with RS, or a low-fat (LF, control) diet for 4 weeks. Cannabinoid CB1 (CB1R) and 5HT1A (5HT1A R) and 5-HT2A (5HT2A R) receptor binding densities are examined. In the hippocampus and hypothalamus, a HF diet significantly increases CB1R binding, while HF + GOS and HF + RS diets prevented this increase. HF diet also increases hippocampal and hypothalamic 5-HT1A R binding, while HF + GOS and HF + RS prevented the alterations. Increased 5-HT2A binding is prevented by HF + GOS and HF + RS in the medial mammillary nucleus.

Conclusions: These results demonstrate that increased CB1R, 5-HT1A R and 5-HT2A R induced by a HF diet can be prevented by GOS and RS supplementation in brain regions involved in cognition and appetite. Therefore, increased fiber intake may have beneficial effects on improving learning and memory, as well as reducing excessive appetite, during the chronic consumption of a HF (standard Western) diet.

Keywords: CB1 receptors; galacto-oligosaccharides; high-fat diet; resistant starch; serotonin receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Appetite / drug effects*
  • Appetite / physiology
  • Brain / drug effects*
  • Brain / metabolism
  • Chemokine CCL2 / blood
  • Cognition Disorders / prevention & control
  • Diet, High-Fat / adverse effects*
  • Dietary Fiber / pharmacology
  • Dietary Supplements
  • Energy Intake / drug effects
  • Male
  • Oligosaccharides / chemistry
  • Oligosaccharides / pharmacology*
  • Rats, Wistar
  • Receptor, Cannabinoid, CB1 / metabolism
  • Receptor, Serotonin, 5-HT1A / metabolism
  • Receptor, Serotonin, 5-HT2A / metabolism
  • Starch / pharmacology*

Substances

  • Ccl2 protein, rat
  • Chemokine CCL2
  • Dietary Fiber
  • Oligosaccharides
  • Receptor, Cannabinoid, CB1
  • Receptor, Serotonin, 5-HT2A
  • Receptor, Serotonin, 5-HT1A
  • Starch