HLA-DQB1*06:02 allele frequency and clinic-polysomnographic features in Saudi Arabian patients with narcolepsy

Sleep Breath. 2019 Mar;23(1):303-309. doi: 10.1007/s11325-018-1717-4. Epub 2018 Sep 5.

Abstract

Background: Narcolepsy is an uncommon neurological disorder characterised by irresistible spells of sleep associated with abnormal rapid eye movement (REM) sleep. The association between narcolepsy and human leukocyte antigen HLA- DQB1*06:02 has been established elsewhere but remains to be investigated among Saudi Arabian patients with narcolepsy.

Methods: A total of 29 Saudi patients with type I or type 2 narcolepsy comprising of 23 (79%) males and 6 (21%) females with a mean age of 17.2 ± 9.6 years were included in this study. Type 1 or type 2 narcolepsy was diagnosed by full polysomnography followed by a multiple sleep latency test in accordance with International Classifications of Sleep Disorders-3 criteria. HLA typing for DQB1 alleles was performed by polymerase chain reaction and hybridization with sequence-specific oligonucleotide probes. Differences in clinical and sleep parameters were compared by univariable analyses. HLA-DQB1*06:02 frequency was systematically compared with the published literature.

Results: Type 1 narcolepsy was diagnosed in 19/29 (65.5%) patients, whereas 10/29 (34.5%) patients had type 2 narcolepsy. DQB1*06:02 was present in 25/29 (86.2%) patients; 15/19 (78.9%) narcolepsy type 1 patients and 10/10 (100%) narcolepsy type 2 patients harboured the DQB1*06:02 allele. REM latency was significantly lower in DQB1*06:02-positive patients compared to DQB1*06:02-negative patients (17.6 ± 32.3 min vs. 106.0 ± 86.0 min; p = 0.025). Epworth Sleepiness Scale scores were significantly higher among type 1 than type 2 narcolepsy patients (19.7 ± 3.2 vs 15.3 ± 3.6; p = 0.02).

Conclusions: DQB1*06:02 allele frequencies among Saudi patients with narcolepsy were consistent with previously published data.

Keywords: Autoimmune disease, DQB1*06:02; Haplotype; Hypersomnia; Sleep; Sleep studies.

MeSH terms

  • Adolescent
  • Adult
  • Arabs / genetics*
  • Female
  • Gene Frequency / genetics*
  • Genetic Predisposition to Disease / genetics
  • HLA-DQ beta-Chains / genetics*
  • Humans
  • Male
  • Narcolepsy / diagnosis
  • Narcolepsy / genetics*
  • Polysomnography*
  • Saudi Arabia
  • Young Adult

Substances

  • HLA-DQ beta-Chains
  • HLA-DQB1 antigen