Flow Cytometry

Amaia Gascue; Juana Merino; Bruno Paiva

doi:10.1016/j.hoc.2018.05.004

Flow Cytometry

Hematol Oncol Clin North Am. 2018 Oct;32(5):765-775. doi: 10.1016/j.hoc.2018.05.004. Epub 2018 Jul 31.

Authors

Amaia Gascue¹, Juana Merino¹, Bruno Paiva²

Affiliations

¹ Department of immunology, Clínica Universidad de Navarra, Av. Pio XII, 36, Pamplona, 31008, Spain.
² Department of immunology, Clínica Universidad de Navarra, Av. Pio XII, 36, Pamplona, 31008, Spain; Centre for Applied Medical Research (CIMA), Pamplona, Spain; Healthcare Research Institute of Navarre (IDISNA), Pamplona, Spain; CIBERONC, Spain. Electronic address: [email protected].

PMID: 30190016
DOI: 10.1016/j.hoc.2018.05.004

Abstract

Multiparameter flow cytometry (MFC) is valuable in the diagnosis and monitoring of most hematological malignancies. Although the assessment of cellular infiltrates in Waldenström macroglobulinemia (WM) relies on morphology and immunohistochemistry grounds, there is evidence pointing to the clinical significance of MFC in this disease. Herein, the authors review immunophenotypic patterns of B-cell development, the antigen profile of the WM clone and its normal B-cell counterpart, the clinical applicability of MFC in the differential diagnosis of immunoglobulin M-secreting lymphoproliferative disorders and monoclonal gammopathies, and its potential role in detecting minimal residual disease and monitoring treatment efficacy in WM.

Keywords: B cells; Flow cytometry; Immunophenotyping; Plasma cells; Waldenström macroglobulinemia.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

B-Lymphocytes* / metabolism
B-Lymphocytes* / pathology
Flow Cytometry / methods*
Hematologic Neoplasms* / blood
Hematologic Neoplasms* / pathology
Humans
Neoplasm, Residual
Waldenstrom Macroglobulinemia* / blood
Waldenstrom Macroglobulinemia* / pathology