Role of Ttca of Citrobacter Werkmanii in Bacterial Growth, Biocides Resistance, Biofilm Formation and Swimming Motility

Gang Zhou; Ying-Si Wang; Hong Peng; Xiao-Mo Huang; Xiao-Bao Xie; Qing-Shan Shi

doi:10.3390/ijms19092644

Role of Ttca of Citrobacter Werkmanii in Bacterial Growth, Biocides Resistance, Biofilm Formation and Swimming Motility

Int J Mol Sci. 2018 Sep 6;19(9):2644. doi: 10.3390/ijms19092644.

Authors

Gang Zhou¹, Ying-Si Wang², Hong Peng³, Xiao-Mo Huang⁴, Xiao-Bao Xie⁵, Qing-Shan Shi⁶

Affiliations

¹ Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China. [email protected].
² Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China. [email protected].
³ Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China. [email protected].
⁴ Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China. [email protected].
⁵ Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China. [email protected].
⁶ Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China. [email protected].

Abstract

To screen, identify and study the genes involved in isothiazolone resistance and biofilm formation in Citrobacter werkmanii strain BF-6. A Tn5 transposon library of approximately 900 mutants of C. werkmanii strain BF-6 was generated and screened to isolate 1,2-benzisothiazolin-3-one (BIT) resistant strains. In addition, the tRNA 2-thiocytidine (32) synthetase gene (ttcA) was deleted through homologous recombination and the resulting phenotypic changes of the ΔttcA mutant were studied. A total of 3 genes were successfully identified, among which ΔttcA mutant exhibited a reduction in growth rate and swimming motility. On the other hand, an increase in biofilms formation in ΔttcA were observed but not with a significant resistance enhancement to BIT. This work, for the first time, highlights the role of ttcA gene of C. werkmanii strain BF-6 in BIT resistance and biofilm formation.

Keywords: Citrobacter werkmanii; Tn5 mutagenesis; biofilm formation; swimming motility; ttcA gene.

MeSH terms

Bacterial Proteins / genetics
Biofilms / drug effects
Biofilms / growth & development*
Citrobacter / drug effects
Citrobacter / physiology*
Disinfectants / pharmacology*
Drug Resistance, Bacterial
Gene Library
Mutagenesis
Phylogeny
Sulfurtransferases / genetics*
Thiazoles / pharmacology

Substances

Bacterial Proteins
Disinfectants
Thiazoles
Sulfurtransferases
1,2-benzisothiazoline-3-one

Abstract

MeSH terms

Substances

Grants and funding