A hyperactive mutant of interferon-regulatory factor 4

Chol Ho Kang; Enno Hartmann; Lisa Menke; Daniel Staudenraus; El-Fadil Abass; Hartmann Raifer; Alekya Porapu; Bärbel Camara; Anne Brüstle; Olaf Pinkenburg; Maria Bieringer; Michael Lohoff

doi:10.1002/eji.201847530

A hyperactive mutant of interferon-regulatory factor 4

Eur J Immunol. 2019 May;49(5):812-815. doi: 10.1002/eji.201847530. Epub 2018 Sep 30.

Affiliations

¹ Institute for Medical Microbiology Hospital Hygiene, Philipps University Marburg, Marburg, Germany.
² Flow Cytometry Core Facility BMFZ¸ Philipps University Marburg, Marburg, Germany.
³ Department of Immunology and Infectious Disease, John Curtin School of Medical Research, Australian National University, Canberra, Australia.

PMID: 30218581
DOI: 10.1002/eji.201847530

Abstract

We found that deletion of the final 30 amino acids of transcription factor IRF4's (interferon-regulatory factor) C-terminus creates hyperactive IRF4. When introduced into IRF4-deficient CD4⁺ or CD8⁺ T cells, more type 17 differentiation was found compared to WT IRF4. Interestingly, Th9 differentiation and Th2-linked IL-13 production were much less altered.

Keywords: IRF4; Mutation; T cell differentiation; Th17; Transcriptional activity.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Interferon Regulatory Factors / genetics*
Interferon Regulatory Factors / metabolism
Mutation*
T-Lymphocyte Subsets / immunology
T-Lymphocyte Subsets / metabolism*

Substances

Interferon Regulatory Factors
interferon regulatory factor-4