Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50

Stem Cell Res. 2018 Oct:32:78-82. doi: 10.1016/j.scr.2018.09.003. Epub 2018 Sep 6.

Abstract

Arrhythmogenic Cardiomyopathy (ACM) is an inherited cardiac disease characterized by arrhythmias and fibro-fatty replacement in the ventricular myocardium. Causative mutations are mainly reported in desmosomal genes, especially in plakophilin2 (PKP2). Here, using a virus-free reprogramming approach, we generated induced pluripotent stem cells (iPSCs) from skin fibroblasts of one ACM patient carrying the frameshift heterozygous PKP2 mutation c.2569_3018del50. The iPSC line (EURACi004-A) showed the typical morphology of pluripotent cells, possessed normal karyotype and exhibited pluripotency markers and trilineage differentiation potential, including cardiomyogenic capability. Thus, this line can represent a human in vitro model to study the molecular basis of ACM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arrhythmias, Cardiac / metabolism
  • Cardiomyopathies / metabolism
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Fibroblasts / cytology*
  • Fibroblasts / metabolism
  • Heterozygote
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Mutation / genetics
  • Plakophilins / genetics*
  • Skin / cytology*

Substances

  • PKP2 protein, human
  • Plakophilins