Functional Imaging with Nucleic-Acid-Based Sensors: Technology, Application and Future Healthcare Prospects

Chembiochem. 2019 Feb 15;20(4):437-450. doi: 10.1002/cbic.201800430. Epub 2018 Nov 20.

Abstract

Timely monitoring and assessment of human health plays a crucial role in maintaining the wellbeing of our advancing society. In addition to medical tools and devices, suitable probe agents are crucial to assist such monitoring, either in passive or active ways (i.e., sensors) through inducible signals. In this review we highlight recent developments in activatable optical sensors based on nucleic acids. Sensing mechanisms and bio-applications of these nucleic acid sensors in ex vivo assays, intracellular or in vivo settings are described. In addition, we discuss the limitations of these sensors and how nanotechnology can complement/enhance sensor properties to promote translation into clinical applications.

Keywords: activatable probes; biosensors; functional imaging; nanotechnology; nucleic acids.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aptamers, Nucleotide / chemistry
  • Biomarkers / metabolism
  • Biosensing Techniques / methods*
  • Contrast Media / chemistry
  • Humans
  • MicroRNAs / metabolism
  • Nanoparticles / chemistry
  • Nanotechnology
  • Nucleic Acids / chemistry*
  • Nucleic Acids / metabolism
  • Whole Body Imaging

Substances

  • Aptamers, Nucleotide
  • Biomarkers
  • Contrast Media
  • MicroRNAs
  • Nucleic Acids