Persistent circulation of genotype D coxsackievirus A2 in mainland of China since 2008

PLoS One. 2018 Sep 20;13(9):e0204359. doi: 10.1371/journal.pone.0204359. eCollection 2018.

Abstract

Coxsackievirus A2 (CV-A2) has emerged as an important etiological agent in the hand, foot, and mouth disease and herpangina pathogen spectrum because of its high global prevalence. In the present study, we investigated the evolutionary dynamics of CV-A2 circulating in China. We analyzed a total of 163 entire VP1 sequences of CV-A2, including 74 sequences generated from the present study and 89 sequences collected from the GenBank database. Phylogenetic analysis based on the entire VP1 nucleotide sequences confirmed the persistent circulation of the predominant genotype D in mainland of China since 2008. Cluster analysis grouped the sequences into two distinct clusters, clusters 1 and 2, with most grouped under cluster 2. After 2012, cluster 1 was gradually replaced by cluster 2. Results of Bayesian Markov chain Monte Carlo analysis suggested that multiple lineages of genotype D were transmitted in mainland of China at an estimated evolutionary rate of 6.32×10(-3) substitutions per site per year, which is consistent with the global evolutionary rate of CV-A2 (5.82×10(-3) substitutions per site per year). Continuous transmission and evolution of CV-A2 resulted in the genetic polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / genetics
  • China
  • Enterovirus / genetics*
  • Enterovirus / isolation & purification*
  • Evolution, Molecular
  • Genotype*
  • Geography
  • Humans
  • Phylogeny

Substances

  • Capsid Proteins

Grants and funding

Funding was provided by National Key Technologies Research and Development Program of National Ministry of Science (2013ZX10004202) to WX and National Key Technologies Research and Development Program of National Ministry of Science (2017ZX10104001) to YZ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.