UBA1/GARS-dependent pathways drive sensory-motor connectivity defects in spinal muscular atrophy

Brain. 2018 Oct 1;141(10):2878-2894. doi: 10.1093/brain/awy237.

Abstract

Deafferentation of motor neurons as a result of defective sensory-motor connectivity is a critical early event in the pathogenesis of spinal muscular atrophy, but the underlying molecular pathways remain unknown. We show that restoration of ubiquitin-like modifier-activating enzyme 1 (UBA1) was sufficient to correct sensory-motor connectivity in the spinal cord of mice with spinal muscular atrophy. Aminoacyl-tRNA synthetases, including GARS, were identified as downstream targets of UBA1. Regulation of GARS by UBA1 occurred via a non-canonical pathway independent of ubiquitylation. Dysregulation of UBA1/GARS pathways in spinal muscular atrophy mice disrupted sensory neuron fate, phenocopying GARS-dependent defects associated with Charcot-Marie-Tooth disease. Sensory neuron fate was corrected following restoration of UBA1 expression and UBA1/GARS pathways in spinal muscular atrophy mice. We conclude that defective sensory motor connectivity in spinal muscular atrophy results from perturbations in a UBA1/GARS pathway that modulates sensory neuron fate, thereby highlighting significant molecular and phenotypic overlap between spinal muscular atrophy and Charcot-Marie-Tooth disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acyl-tRNA Synthetases / metabolism*
  • Animals
  • Ganglia, Spinal / metabolism
  • Ganglia, Spinal / pathology
  • Gene Expression Regulation / physiology
  • HEK293 Cells
  • Humans
  • Mice
  • Motor Neurons / metabolism
  • Motor Neurons / pathology
  • Muscular Atrophy, Spinal / metabolism
  • Muscular Atrophy, Spinal / pathology*
  • Neural Pathways / metabolism
  • Neural Pathways / pathology*
  • Sensory Receptor Cells / metabolism
  • Sensory Receptor Cells / pathology
  • Signal Transduction / physiology
  • Spinal Cord / metabolism
  • Spinal Cord / pathology
  • Ubiquitin-Activating Enzymes / metabolism*

Substances

  • Amino Acyl-tRNA Synthetases
  • Ubiquitin-Activating Enzymes