The identification of induction chemo-sensitivity genes of laryngeal squamous cell carcinoma and their clinical utilization

Eur Arch Otorhinolaryngol. 2018 Nov;275(11):2773-2781. doi: 10.1007/s00405-018-5134-x. Epub 2018 Sep 28.

Abstract

Purpose: To identify potential molecular markers for induction chemotherapy of Laryngeal squamous cell carcinoma (LSCC).

Methods: Differently expressed genes between chemo-sensitive group (seven cases) and chemo-insensitive (five cases) group after induction chemotherapy by TPF were identified by microarrays. Bayes network and Random forest analyses were employed to identify core genes for induction chemotherapy. The diagnostic value of these core genes was also evaluated by ROC analysis.

Results: Six genes (SPP1, FOLR3, KYNU, LOC653219, ADH7 and XAGE1A) are highly expressed, while seven gene (CADM1, NDUFA4L2, CCND2, RARRES3, ERAP2, LYD6 and CNTNAP2) present significantly low expression. Among these genes, genes CADM1, FOLR3, KYNU, and CNTNAP2 are core candidates for LSCC chemo-sensitivity. And that the low expression of CADM1 may result in chemo-sensitivity, which leads to high expression of gene FOLR3 and KYNU, and low expression of gene CNTNAP2. Besides, ROC analysis shows that these four genes exhibit effective diagnostic value for induction chemo-sensitivity.

Conclusions: CADM1 may be a potential molecular marker for LSCC induction chemotherapy, while CADM1, FOLR3, KYNU, and CNTNAP2 may provide essential guidance for LSCC diagnosis and follow-up treatment strategies.

Keywords: Chemo-sensitivity; Induction chemotherapy; Laryngeal squamous cell carcinoma; Molecular marker.

MeSH terms

  • Aged
  • Carcinoma, Squamous Cell / drug therapy*
  • Carcinoma, Squamous Cell / genetics*
  • Carrier Proteins / genetics
  • Cell Adhesion Molecule-1 / genetics
  • Drug Resistance, Neoplasm
  • Female
  • Gene Expression Profiling
  • Genetic Markers
  • Humans
  • Induction Chemotherapy*
  • Laryngeal Neoplasms / drug therapy*
  • Laryngeal Neoplasms / genetics*
  • Male
  • Membrane Proteins / genetics
  • Microarray Analysis
  • Middle Aged
  • Nerve Tissue Proteins / genetics
  • RNA, Complementary / metabolism

Substances

  • CADM1 protein, human
  • CNTNAP2 protein, human
  • Carrier Proteins
  • Cell Adhesion Molecule-1
  • FOLR3 protein, human
  • Genetic Markers
  • Membrane Proteins
  • Nerve Tissue Proteins
  • RNA, Complementary