Abstract
Cloned cDNA encoding the alpha-subunit of the adenylate cyclase-stimulating G-protein (Gs), carried by a simian virus 40 vector, has been introduced into the cyc- variant of S49 lymphoma cells by electroporation. In contrast to untransfected cys- cells, clones transformed with the cDNA exhibit an increase in intracellular cyclic AMP concentration in response to a beta-adrenergic agonist.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenylyl Cyclases / metabolism
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Animals
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Cell Line
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Cloning, Molecular*
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Cyclic AMP / metabolism
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DNA / metabolism*
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GTP-Binding Proteins / genetics*
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Genes*
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Isoproterenol / pharmacology
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Lymphoma / enzymology
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Macromolecular Substances
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Mice
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Nucleic Acid Hybridization
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Plasmids
Substances
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Macromolecular Substances
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DNA
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Cyclic AMP
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GTP-Binding Proteins
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Adenylyl Cyclases
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Isoproterenol