Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 496 adults from San Diego, California, USA

Eugene Moore; Alba Grifoni; Daniela Weiskopf; Veronique Schulten; Cecilia S Lindestam Arlehamn; Michael Angelo; John Pham; Shay Leary; John Sidney; David Broide; April Frazier; Elizabeth Phillips; Simon Mallal; Steven J Mack; Alessandro Sette

doi:10.1016/j.humimm.2018.09.008

Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 496 adults from San Diego, California, USA

Hum Immunol. 2018 Dec;79(12):821-822. doi: 10.1016/j.humimm.2018.09.008. Epub 2018 Sep 29.

Authors

Affiliations

¹ Division of Vaccine Discovery, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA. Electronic address: [email protected].
² Division of Vaccine Discovery, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA.
³ Institute for Immunology and Infectious Diseases, Murdoch University, Perth, Western Australia 6150, Australia.
⁴ Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA.
⁵ Institute for Immunology and Infectious Diseases, Murdoch University, Perth, Western Australia 6150, Australia; Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
⁶ Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA.
⁷ Division of Vaccine Discovery, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA; Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA.

Abstract

DNA sequence-based typing at the HLA-A, -B, -C, -DPB1, -DQA1, -DQB1, and -DRB1 loci was performed on 496 healthy adult donors from San Diego, California, to characterize allele frequencies in support of studies of T cell responses to common allergens. Deviations from Hardy Weinberg proportions were detected at each locus except A and C. Several alleles were found in more than 15% of individuals, including the class II alleles DPB1∗02:01, DPB1∗04:01, DQA1∗01:02, DQA1∗05:01, DQB1∗03:01, and the class I allele A∗02:01. Genotype data will be available in the Allele Frequencies Net Database (AFND 3562).

Keywords: Allergy; HLA alleles; HLA typing; San Diego, California.

MeSH terms

Adolescent
Adult
Alleles
California
Female
Gene Frequency
Genotype
Genotyping Techniques / methods
HLA-A Antigens / genetics*
HLA-B Antigens / genetics*
HLA-C Antigens / genetics*
HLA-DP Antigens / genetics*
HLA-DQ Antigens / genetics*
HLA-DR Antigens / genetics*
Histocompatibility Testing / methods
Humans
Linkage Disequilibrium
Male
Middle Aged
Sequence Analysis, DNA / methods
T-Lymphocytes / immunology
T-Lymphocytes / metabolism
Young Adult

Substances

HLA-A Antigens
HLA-B Antigens
HLA-C Antigens
HLA-DP Antigens
HLA-DQ Antigens
HLA-DR Antigens

Abstract

MeSH terms

Substances

Grants and funding