Effect of Long-Term Exercise Training on lncRNAs Expression in the Vascular Injury of Insulin Resistance

J Cardiovasc Transl Res. 2018 Dec;11(6):459-469. doi: 10.1007/s12265-018-9830-0. Epub 2018 Oct 9.

Abstract

LncRNA microarray analysis was applied to investigate the exercise effect on the global differential expressions of lncRNA and mRNA in aorta endothelium in insulin resistance. Twenty-four male C57BL/6 J mice were randomly divided into three groups: control group (n = 8), high-fat diet group (n = 8), and high-fat diet plus exercise training group (n = 8). An lncRNA microarray analysis was applied to investigate the global differential expressions of lncRNA and mRNA in aorta endothelium among three groups and the results were further verified by qPCR. Hypergeometric distribution analysis was applied to reveal the possible signaling. Exercise might alleviate the vascular injury of IR via FR030200-Col3A1, FR402720-Rnd1, and FR030200/FR402720-Rnd3 signalings in cytoskeletal rearrangement pathway; E2F1-FR030200/FR402720-Nnat and FR030200/ FR402720-Fam46a signalings in anti-inflammation pathway. This study identified a panel of dysregulated lncRNAs and mRNAs that might serve as potential biomarkers relevant to the vascular injury in insulin resistance induced by high-fat diet.

Keywords: Exercise; Insulin resistance; Vascular injury; lncRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta / metabolism*
  • Aorta / pathology
  • Aortic Diseases / genetics
  • Aortic Diseases / metabolism
  • Aortic Diseases / pathology
  • Aortic Diseases / prevention & control*
  • Diet, High-Fat
  • Disease Models, Animal
  • Exercise Therapy / methods*
  • Gene Expression Profiling / methods
  • Gene Expression Regulation
  • Insulin Resistance* / genetics
  • Male
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis
  • Physical Conditioning, Animal / methods*
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Signal Transduction
  • Time Factors
  • Transcriptome

Substances

  • RNA, Long Noncoding
  • RNA, Messenger