Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

Methods Mol Biol. 2019:1879:153-163. doi: 10.1007/7651_2018_151.

Abstract

Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.

Keywords: Hematopoietic stem cell; MACS; Skin stem cell; Spermatogonial stem cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD34 / metabolism
  • Cell Separation / methods*
  • Hematopoietic Stem Cell Transplantation / methods
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Immunomagnetic Separation / methods
  • Magnetics / methods
  • Mice
  • Mice, Inbred C57BL
  • Stem Cells / cytology*
  • Stem Cells / metabolism
  • Thy-1 Antigens / metabolism

Substances

  • Antigens, CD34
  • Thy-1 Antigens