Large-Scale Preparation of Extracellular Vesicles Enriched with Specific microRNA

Tissue Eng Part C Methods. 2018 Nov;24(11):637-644. doi: 10.1089/ten.TEC.2018.0249. Epub 2018 Nov 2.

Abstract

This article describes a method for producing microRNA (miRNA)-enriched extracellular vesicles in large quantities. It enables in vivo delivery of specific miRNA for therapeutic applications.

Keywords: extracellular vesicle; large-scale production; miRNA-133a-3p; miRNA-202-5p; microRNA.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Extracellular Vesicles / metabolism*
  • Female
  • Gene Transfer Techniques*
  • HEK293 Cells
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / administration & dosage*
  • MicroRNAs / blood
  • MicroRNAs / genetics
  • MicroRNAs / isolation & purification*

Substances

  • MIRN133 microRNA, human
  • MIRN202 microRNA, human
  • MicroRNAs