Cheiromyia carolina Limeira-de-Oliveira Brooks sp. nov. and C. nordestina Limeira-de-Oliveira Cumming sp. nov. are described from Brazil, and C. fuscipennis Pollet Brooks sp. nov. is described from the Mitaraka Mountains in southwestern French Guiana. New distribution records are reported for C. brevitarsis Brooks, C. palmaticornis (Parent) and C. pennaticornis (Parent), and a revised key to males of the eight known species of Cheiromyia Dyte, 1980 is provided. The female of C. pennaticornis is also described for the first time.
Keywords: Diptera, Empidoidea, Dolichopodidae, Cheiromyia, Neotropical, Brazil, French Guiana, new species, key.