Abstract
SNAP-29 is expressed throughout the life cycle of fruit fly and exhibits wide tissue distribution patterns. Unlike other SNAP-25-like proteins (i.e., SNAP-25, SNAP-23/24, and SNAP-47) which primarily support exocytosis at the plasma membrane, SNAP-29 regulates various intracellular trafficking events, by partnering with proteins active in both exocytosis and endocytosis. Here we describe the protocol to localize SNAP-29 in early embryos, imaginal discs from third instar larva, and immortalized S2 cells via immunofluorescence microscopy.
Keywords:
Drosophila; SNAP-29; Ubisnap; usnp.
MeSH terms
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Animals
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Cell Line
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Cell Membrane / metabolism*
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Drosophila Proteins / chemistry
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / metabolism
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Embryo, Nonmammalian / diagnostic imaging
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Embryo, Nonmammalian / metabolism
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Endocytosis
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Exocytosis
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Fluorescent Dyes / chemistry
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Imaginal Discs / diagnostic imaging
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Imaginal Discs / metabolism
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Larva / metabolism
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods
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Models, Animal
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SNARE Proteins / chemistry
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SNARE Proteins / metabolism*
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Single Molecule Imaging / instrumentation
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Single Molecule Imaging / methods*
Substances
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Drosophila Proteins
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Fluorescent Dyes
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SNARE Proteins
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Snap29 protein, Drosophila