Objective: To elucidate the effect of taurine on neurotoxicity induced by Mn by investigating cell cycle and apoptosis in manganese exposed rats. Methods: 156 healthy male SD rats were randomly divided into 1 control group, 3 manganese exposure groups (10, 15, and 20 mg/kg respectively) , and 9 intervened groups based on orthogonal design, with 12 rats in each group. After 12 weeks of exposure, all rats were decapitated and striatums were removed, cell cycle was analyzed by flow cytometry, the apoptosis was detected by TUNEL, level of Mn was determined. Results: The striatum apoptosis index of the 3 dose groups exposed to Mn were significantly higher than control group (P<0.05) . The striatum apoptosis index of the 9 intervened groups were significantly higher than control group (P<0.05) . 150 and 200 mg/kg of taurine could decrease apoptosis index of the group exposed to 10、15、20 mg/kg of Mn (P<0.05) . The striatum Mn content of the 3 dose groups exposed to Mn were significantly higher than that of the control group (P<0.05) . The G0/G1 proportion of the 3 dose groups exposed to Mn were significantly lower than that of the control group (P<0.05) , the S proportion of the 3 dose groups exposed to Mn were significantly higher than that of the control group (P<0.05) . Conclusion: Mn could cause cell cycle arrest to S, increase level of apoptosis in striatum, to a certain extent, taurine can protect neurons from apoptosis induced by Mn.
目的： 观察细胞周期和细胞凋亡的变化，探讨牛磺酸（Tau）对锰染毒大鼠神经毒性的影响。 方法： 取SD健康雄性大鼠156只,随机分为对照组，10、15、20 mg/kg 3个剂量的锰染毒组和Tau干预组（100、150、200 mg/kg Tau），每组12只大鼠。干预12周后，开颅分离出大鼠纹状体，分别进行Tunel染色、锰含量水平测定、流式细胞仪测定细胞周期。 结果： 锰染毒组和Tau干预组纹状体细胞凋亡指数均高于对照组，差异有统计学意义（P<0.05）。150、200 mg/kg Tau干预对10、15、20 mg/kg锰染毒组大鼠纹状体细胞凋亡指数有降低作用，差异有统计学意义（P<0.05）；各染锰组和Tau干预组纹状体锰含量均高于对照组，差异均有统计学意义（均P<0.05）；锰染毒组G0/G1期细胞所占的比例均比空白对照组低，S期细胞比例均比空白对照组高，差异均有统计学意义（均P<0.05）。 结论： 锰可将大鼠纹状体神经组织细胞的细胞周期阻滞于S期，致大鼠纹状体神经组织细胞出现程度不同的细胞凋亡，牛磺酸在一定程度上对锰诱导的大鼠纹状体神经组织细胞凋亡有保护作用。.
Keywords: Apoptosis; Cell Cycle; Manganese; Rat; Taurine.