Mitochondrial Dysregulation and Impaired Autophagy in iPSC-Derived Dopaminergic Neurons of Multiple System Atrophy

Stem Cell Reports. 2018 Nov 13;11(5):1185-1198. doi: 10.1016/j.stemcr.2018.09.007. Epub 2018 Oct 18.

Abstract

Multiple system atrophy (MSA) is a progressive neurodegenerative disease that affects several areas of the CNS, whose pathogenesis is still widely unclear and for which an effective treatment is lacking. We have generated induced pluripotent stem cell-derived dopaminergic neurons from four MSA patients and four healthy controls and from two monozygotic twins discordant for the disease. In this model, we have demonstrated an aberrant autophagic flow and a mitochondrial dysregulation involving respiratory chain activity, mitochondrial content, and CoQ10 biosynthesis. These defective mechanisms may contribute to the onset of the disease, representing potential therapeutic targets.

Keywords: MSA; autophagy; dopaminergic neurons; induced pluripotent stem cells; mitochondria; multiple system atrophy; neurodegeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Autophagy*
  • Biomarkers / metabolism
  • Case-Control Studies
  • Dopaminergic Neurons / pathology*
  • Female
  • Humans
  • Induced Pluripotent Stem Cells / pathology*
  • Male
  • Middle Aged
  • Mitochondria / pathology*
  • Multiple System Atrophy / pathology*
  • Young Adult

Substances

  • Biomarkers