Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar-to-Ductal Metaplasia

Jun Liu; Pei Wang

doi:10.1007/978-1-4939-8879-2_5

Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar-to-Ductal Metaplasia

Methods Mol Biol. 2019:1882:55-62. doi: 10.1007/978-1-4939-8879-2_5.

Authors

Jun Liu¹, Pei Wang²

Affiliations

¹ Department of Cell Systems and Anatomy, The University of Texas Health San Antonio, San Antonio, TX, USA.
² Department of Cell Systems and Anatomy, The University of Texas Health San Antonio, San Antonio, TX, USA. [email protected].

PMID: 30378043
DOI: 10.1007/978-1-4939-8879-2_5

Abstract

Acinar-to-ductal metaplasia may play important roles in the development of various pancreatic diseases. Here, we describe a method to induce ADM in primary human cells in 3D culture. We developed a flow cytometry lineage tracing strategy to identify and sort viable acinar, ductal, and acinar-derived ductal-like cells for further molecular and functional analysis.

Keywords: 3D culture; Acinar-to-ductal metaplasia; Flow cytometry; Human exocrine pancreatic cells; Lineage tracing.

MeSH terms

Acinar Cells / metabolism
Acinar Cells / pathology*
Carcinoma, Pancreatic Ductal / pathology
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods
Cell Separation / instrumentation
Cell Separation / methods*
Cell Transformation, Neoplastic / pathology
Cells, Cultured
Flow Cytometry / instrumentation
Flow Cytometry / methods*
Humans
Metaplasia / pathology
Pancreas, Exocrine / cytology
Pancreas, Exocrine / metabolism
Pancreas, Exocrine / pathology*
Pancreatic Ducts / cytology
Pancreatic Ducts / pathology*
Pancreatic Neoplasms / pathology
Spheroids, Cellular
Transforming Growth Factor beta1 / metabolism

Substances

TGFB1 protein, human
Transforming Growth Factor beta1