Abstract
The effect of gamma-aminobutyric acid (GABA)receptor stimulation on norepinephrine (NE)-induced metabolism of polyphosphoinositides (PIPs) was studied in rat hippocampal slices. Inositol phosphates (IPs), PIPs, and phosphatidic acid were measured. NE induced formation of IPs and phosphatidic acid in a dose-dependent manner with an EC50 of 4.5 microM. GABA, 3-aminopropanesulphonic acid (3APS) and muscimol did not affect PIPs breakdown, but they strongly increased (greater than 100%) PIPs metabolism induced by 1 microM NE. Their action was antagonized by bicuculline (10 microM). We discuss the implications of these findings in hippocampal neurotransmission.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bicuculline / pharmacology
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Hippocampus / drug effects
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Hippocampus / metabolism*
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In Vitro Techniques
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Inositol Phosphates / metabolism
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Male
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Muscimol / pharmacology
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Norepinephrine / pharmacology*
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Phosphatidic Acids / metabolism
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Phosphatidylinositol Phosphates
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Phosphatidylinositols / metabolism*
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Rats
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Rats, Inbred Strains
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Receptors, GABA-A / drug effects
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Receptors, GABA-A / physiology*
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Taurine / analogs & derivatives
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Taurine / pharmacology
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gamma-Aminobutyric Acid / pharmacology
Substances
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Inositol Phosphates
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Phosphatidic Acids
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Phosphatidylinositol Phosphates
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Phosphatidylinositols
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Receptors, GABA-A
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Taurine
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Muscimol
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gamma-Aminobutyric Acid
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tramiprosate
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Norepinephrine
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Bicuculline