Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata

Yirui Wang; Shaowei Han; Ruofei Li; Baosong Cui; Xianjie Ma; Xinzhu Qi; Qi Hou; Mingbao Lin; Jinye Bai; Shuai Li

doi:10.1016/j.ijbiomac.2018.10.201

Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata

Int J Biol Macromol. 2019 Feb 1:122:628-635. doi: 10.1016/j.ijbiomac.2018.10.201. Epub 2018 Nov 2.

Authors

Yirui Wang¹, Shaowei Han², Ruofei Li³, Baosong Cui⁴, Xianjie Ma⁵, Xinzhu Qi⁶, Qi Hou⁷, Mingbao Lin⁸, Jinye Bai⁹, Shuai Li¹⁰

Affiliations

¹ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
² State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
³ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
⁴ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
⁵ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
⁶ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
⁷ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
⁸ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
⁹ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].
¹⁰ State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: [email protected].

PMID: 30391591
DOI: 10.1016/j.ijbiomac.2018.10.201

Abstract

Two water-soluble polysaccharides, BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata. The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively. The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively. The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues. BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.

MeSH terms

Animals
Immunologic Factors / chemistry
Immunologic Factors / pharmacology
Leukocyte Count
Methylation
Mice
Molecular Weight
Monosaccharides / analysis
Orchidaceae / chemistry*
Plant Extracts / chemistry*
Plant Extracts / pharmacology*
Plant Tubers / chemistry*
Polysaccharides / chemistry*
Polysaccharides / pharmacology*
Thymus Gland / immunology

Substances

Immunologic Factors
Monosaccharides
Plant Extracts
Polysaccharides