Rapid Capture and Analysis of Airborne Staphylococcus aureus in the Hospital Using a Microfluidic Chip

Xiran Jiang; Yingchao Liu; Qi Liu; Wenwen Jing; Kairong Qin; Guodong Sui

doi:10.3390/mi7090169

Rapid Capture and Analysis of Airborne Staphylococcus aureus in the Hospital Using a Microfluidic Chip

Micromachines (Basel). 2016 Sep 15;7(9):169. doi: 10.3390/mi7090169.

Authors

Xiran Jiang¹, Yingchao Liu², Qi Liu³, Wenwen Jing⁴, Kairong Qin⁵, Guodong Sui⁶

Affiliations

¹ Department of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China. [email protected].
² Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. [email protected].
³ Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China. [email protected].
⁴ Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China. [email protected].
⁵ Department of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China. [email protected].
⁶ Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China. [email protected].

Abstract

In this study we developed a microfluidic chip for the rapid capture, enrichment and detection of airborne Staphylococcus (S.) aureus. The whole analysis took about 4 h and 40 min from airborne sample collection to loop-mediated isothermal amplification (LAMP), with a detection limit down to about 27 cells. The process did not require DNA purification. The chip was validated using standard bacteria bioaerosol and was directly used for clinical airborne pathogen sampling in hospital settings. This is the first report on the capture and analysis of airborne S. aureus using a novel microfluidic technique, a process that could have a very promising platform for hospital airborne infection prevention (HAIP).

Keywords: airborne bacteria; bioaerosols; microfluidics.