Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth

Nat Commun. 2018 Nov 8;9(1):4680. doi: 10.1038/s41467-018-06861-3.

Abstract

Drought represents a major threat to food security. Mechanistic data describing plant responses to drought have been studied extensively and genes conferring drought resistance have been introduced into crop plants. However, plants with enhanced drought resistance usually display lower growth, highlighting the need for strategies to uncouple drought resistance from growth. Here, we show that overexpression of BRL3, a vascular-enriched member of the brassinosteroid receptor family, can confer drought stress tolerance in Arabidopsis. Whereas loss-of-function mutations in the ubiquitously expressed BRI1 receptor leads to drought resistance at the expense of growth, overexpression of BRL3 receptor confers drought tolerance without penalizing overall growth. Systematic analyses reveal that upon drought stress, increased BRL3 triggers the accumulation of osmoprotectant metabolites including proline and sugars. Transcriptomic analysis suggests that this results from differential expression of genes in the vascular tissues. Altogether, this data suggests that manipulating BRL3 expression could be used to engineer drought tolerant crops.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / physiology*
  • Droughts*
  • Gene Expression Regulation, Plant
  • Metabolome
  • Mutation / genetics
  • Osmotic Pressure
  • Plant Development* / genetics
  • Plant Roots / genetics
  • Plant Roots / physiology
  • Plant Vascular Bundle / metabolism*
  • Plants, Genetically Modified
  • Receptors, Cell Surface / metabolism*
  • Stress, Physiological / genetics
  • Transcription, Genetic
  • Tropism

Substances

  • BRL3 protein, Arabidopsis
  • Receptors, Cell Surface