Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA

Diagn Microbiol Infect Dis. 2019 Mar;93(3):188-190. doi: 10.1016/j.diagmicrobio.2018.10.008. Epub 2018 Oct 16.

Abstract

This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.

Keywords: Bacteria; Cerebrospinal; Detection; Meningitis; Real-time PCR; Triplex.

Publication types

  • Comparative Study

MeSH terms

  • Genes, Bacterial / genetics
  • Haemophilus influenzae / genetics*
  • Haemophilus influenzae / isolation & purification
  • Humans
  • Meningitis, Bacterial / cerebrospinal fluid
  • Meningitis, Bacterial / diagnosis*
  • Meningitis, Bacterial / microbiology
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction*
  • Neisseria meningitidis / genetics*
  • Neisseria meningitidis / isolation & purification
  • Real-Time Polymerase Chain Reaction*
  • Sensitivity and Specificity
  • Streptococcus pneumoniae / genetics*
  • Streptococcus pneumoniae / isolation & purification