Spread of GES-5 carbapenemase-producing Pseudomonas aeruginosa clinical isolates in Japan due to clonal expansion of ST235

Tomomi Hishinuma; Tatsuya Tada; Kyoko Kuwahara-Arai; Norio Yamamoto; Masahiro Shimojima; Teruo Kirikae

doi:10.1371/journal.pone.0207134

Spread of GES-5 carbapenemase-producing Pseudomonas aeruginosa clinical isolates in Japan due to clonal expansion of ST235

PLoS One. 2018 Nov 19;13(11):e0207134. doi: 10.1371/journal.pone.0207134. eCollection 2018.

Authors

Tomomi Hishinuma¹, Tatsuya Tada¹, Kyoko Kuwahara-Arai¹, Norio Yamamoto¹, Masahiro Shimojima², Teruo Kirikae¹

Affiliations

¹ Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
² BML Inc., Kawagoe, Saitama, Japan.

Abstract

The first outbreak in Japan of GES-5 carbapenemase-producing Pseudomonas aeruginosa occurred in a long-term care facility in 2014. To assess the spread of GES-5 producing P. aeruginosa clinical isolates in medical settings in Japan, 1,476 carbapenem-resistant P. aeruginosa isolates obtained from 2012 to 2016 were characterized. Of these 1,476 isolates, 104 (7.0%) harbored blaGES-5. Southern blotting revealed that the blaGES-5 was located on the chromosome. The isolation rates of these GES-5 producers increased significantly every year, from 2.0% (6 of 295) in 2012 to 2.8% (8 of 283) in 2013 to 5.3% (16 of 303) in 2014 to 9.7% (29 of 300) in 2015 to 15.3% (45 of 295) in 2016. Of the 104 GES-5 producers, 102 belonged to clonal complex (CC) 235, including 99 belonging to ST235 and three belonging to ST2233). Whole genome sequence analysis revealed that CC235 P. aeruginosa harboring blaGES-5 spread in a clonal manner. These results indicate that these GES-5 producing CC235 P. aeruginosa clinical isolates have spread in medical settings throughout Japan.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
DNA, Bacterial
Drug Resistance, Multiple, Bacterial / genetics*
Drug Resistance, Multiple, Bacterial / physiology
Evolution, Molecular
Geography, Medical
Humans
Japan / epidemiology
Molecular Epidemiology
Phylogeny
Pseudomonas Infections / epidemiology*
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / genetics*
Pseudomonas aeruginosa / isolation & purification
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Bacterial Proteins
DNA, Bacterial
beta-Lactamases
carbapenemase

Grants and funding

The study was supported by grants from Research Program on Emerging and Re-emerging Infectious Diseases from AMED (18fk0108061) to TK (https://www.amed.go.jp/en/program/list/01/06/002.html) and JSPS KAKENHI Grant Number 18K07120 to TT. BML Inc. provided support in the form of salaries for author Masahiro Shimojima, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.