Association between genetic polymorphisms in long non-coding RNAs and pancreatic cancer risk

Cancer Biomark. 2019;24(1):117-123. doi: 10.3233/CBM-181959.

Abstract

Background: Long non-coding RNAs (lncRNAs) are emerging as candidate biomarkers of cancer, having regulatory functions in both oncogenic and tumor-suppressive pathways. Concerning pancreatic cancer (PC), deregulation of lncRNAs involved in tumor initiation, invasion, and metastasis seem to play a key role. However, data is scarce about regulatory mechanism of lncRNA expression.

Objective: The aim of our study was to investigate the contribution of two lncRNAs polymorphisms (rs1561927 and rs4759313 of PVT1 and HOTAIR, respectively) in PC susceptibility.

Methods: A case-control study was conducted analysing rs1561927 and rs4759313 polymorphisms using DNA collected in a population-based case-control study of pancreatic cancer (111 pancreatic ductal adenocarcinoma cases (PDAC), 56 pancreatic neuroendocrine tumor (PNET), and 125 healthy controls).

Results: Regarding the PVT1 rs1561927 polymorphism the G allele was significantly overrepresented in both PDAC and PNET patients compared to the controls, while the presence of the HOTAIR rs4759314 G allele was found to be overrepresented in the PNET patients only compared to the controls. The PVT1 rs1561927 AG/GG genotypes were associated with poor overall survival in PDAC patients.

Conclusions: Our results suggested that polymorphisms of these two lncRNA polymorphisms implicated in pancreatic carcinogenesis. Further large-scale and functional studies are needed to confirm our results.

Keywords: Long non-coding RNA; pancreatic ductal adenocarcinoma; pancreatic neuroendocrine tumors; polymorphisms.

MeSH terms

  • Adult
  • Aged
  • Alleles
  • Case-Control Studies
  • Cell Line, Tumor
  • Female
  • Gene Frequency
  • Genetic Association Studies
  • Genetic Predisposition to Disease*
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Grading
  • Neoplasm Staging
  • Odds Ratio
  • Pancreatic Neoplasms / genetics*
  • Polymorphism, Genetic*
  • RNA, Long Noncoding / genetics*
  • Risk Factors

Substances

  • RNA, Long Noncoding