Comparison of adipose tissue derived genes in endogenous Cushing's syndrome versus diet-induced obesity

Endokrynol Pol. 2019;70(2):131-134. doi: 10.5603/EP.a2018.0091. Epub 2018 Nov 27.

Abstract

Introduction: Dysregulation of adipokine secretion and action is a characteristic feature of obesity and a key clinical feature of Cushing's syndrome (CS). We have investigated whether endogenous glucocorticoid excess influences adipose tissue-derived gene expression.

Material and methods: mRNA expression of adipokines; adiponectin, resistin, tumour necrosis factor-a, interleukin-6 (IL-6), angiotensinogen (AGT), plasminogen activator inhibitor type 1, retinol binding protein 4, visfatin, and cystatin C was assessed by quantitative real-time RT-PCR in visceral adipose tissue removed during abdominal surgery of eight patients with CS, and six control patients.

Results: We did not find any significant difference in the investigated genes; however, the almost significant overexpression of AGT and underexpression of IL-6 might be noteworthy (p = 0.06 in both cases).

Conclusions: No significant differences were found in the expression of the investigated genes known as cardiometabolic risk factors. This indicates that there are no major differences between endogenous hypercortisolism or diet-induced obesity regarding the expression of adipokines involved in cardiometabolic disorders. However, the difference in AGT and IL-6 expression might be included in pathways affecting fat distribution in CS.

Keywords: Cushing’s syndrome; adipokines; glucocorticoids; obesity; visceral adipose tissue.

MeSH terms

  • Adipokines / metabolism*
  • Adipose Tissue / metabolism*
  • Adult
  • Case-Control Studies
  • Cushing Syndrome / metabolism*
  • Female
  • Humans
  • Intra-Abdominal Fat / metabolism
  • Male
  • Obesity, Abdominal / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Adipokines