Abstract
Solanaceous medicinal plants produce tropane alkaloids (TAs). We discovered a novel gene from Atropa belladonna, AbPPAR, which encodes a phenylpyruvic acid reductase required for TA biosynthesis. AbPPAR was specifically expressed in root pericycles and endodermis. AbPPAR was shown to catalyze reduction of phenylpyruvic acid to phenyllactic acid, a precursor of TAs. Suppression of AbPPAR disrupted TA biosynthesis through reduction of phenyllactic acid levels. In summary, we identified a novel enzyme involved in TA biosynthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / biosynthesis*
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Alkaloids / chemistry
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Atropa belladonna / chemistry
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Atropa belladonna / metabolism
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Molecular Structure
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Oxidoreductases / chemistry
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Oxidoreductases / isolation & purification
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Oxidoreductases / metabolism*
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Phenylpyruvic Acids / chemistry
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Phenylpyruvic Acids / isolation & purification
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Phenylpyruvic Acids / metabolism*
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Tropanes / chemistry
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Tropanes / metabolism*
Substances
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Alkaloids
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Phenylpyruvic Acids
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Tropanes
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Oxidoreductases
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phenylpyruvic acid