Escherichia coli NGF-1, a Genetically Tractable, Efficiently Colonizing Murine Gut Isolate

Marika Ziesack; Michiel A P Karrenbelt; Johannes Bues; Elena Schaefer; Pamela Silver; Jeffrey Way

doi:10.1128/MRA.01416-18

Escherichia coli NGF-1, a Genetically Tractable, Efficiently Colonizing Murine Gut Isolate

Microbiol Resour Announc. 2018 Dec 6;7(22):e01416-18. doi: 10.1128/MRA.01416-18. eCollection 2018 Dec.

Authors

Marika Ziesack^#^{1

2}, Michiel A P Karrenbelt^#^{1

2}, Johannes Bues^#^{1

2}, Elena Schaefer^{1

2}, Pamela Silver^{1

2}, Jeffrey Way^{1

2}

Affiliations

¹ Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA.
² Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.

^# Contributed equally.

Abstract

The genome of the murine commensal strain Escherichia coli NGF-1 contains a 5.03-Mbp chromosome and plasmids of 40.2 kbp and 8.56 kbp. NGF-1 efficiently colonizes the mouse gut and is genetically tractable. The genome sequence reported here facilitates genetic engineering and research in mouse models of healthy and diseased intestine.