Closed Genome Sequences of Two Clostridium botulinum Strains Obtained by Nanopore Sequencing

Narjol Gonzalez-Escalona; Julie Haendiges; Jesse D Miller; Shashi K Sharma

doi:10.1128/MRA.01075-18

Closed Genome Sequences of Two Clostridium botulinum Strains Obtained by Nanopore Sequencing

Microbiol Resour Announc. 2018 Sep 6;7(9):e01075-18. doi: 10.1128/MRA.01075-18. eCollection 2018 Sep.

Authors

Narjol Gonzalez-Escalona¹, Julie Haendiges^{1

2}, Jesse D Miller², Shashi K Sharma¹

Affiliations

¹ Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA.
² NSF International, Applied Research Center, Ann Arbor, Michigan, USA.

Abstract

Here we report the genome sequences of two toxin-producing Clostridium botulinum strains, one environmental sample (83F) and one clinical sample (CDC51232). The genomes were closed by a combination of long-read and short-read sequencing. The strains belong to C. botulinum sequence type 4 (ST4) and ST7, respectively.