OVA-Induced Allergic Airway Inflammation Mouse Model

Methods Mol Biol. 2019:1916:297-301. doi: 10.1007/978-1-4939-8994-2_28.

Abstract

Asthma is a worldwide public health issue, affecting the sufferer's quality of life. Many researchers are extensively studying the cellular processes involved in the affected airways. Experimental asthma using animals has been performed for a long time, mainly applying murine models due to well-known advantages. The aim of this study is to present an allergic airway inflammation protocol in mice. Basically, the allergic airway inflammation is induced by intraperitoneal sensitization and intratracheal challenge with ovalbumin (OVA). The model provided here mimics acute asthma characteristics including excessive mucus production, airway hyperresponsiveness, and eosinophilic airway inflammation.

Keywords: Airway inflammation; Allergy; Asthma; Ovalbumin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / immunology
  • Animals
  • Asthma / genetics
  • Asthma / immunology*
  • Asthma / pathology
  • Bronchoalveolar Lavage Fluid / immunology
  • Disease Models, Animal*
  • Female
  • Humans
  • Inflammation / genetics
  • Inflammation / immunology*
  • Inflammation / pathology
  • Lung / immunology
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin / adverse effects
  • Ovalbumin / immunology
  • Respiratory Hypersensitivity / genetics
  • Respiratory Hypersensitivity / immunology*
  • Respiratory Hypersensitivity / pathology

Substances

  • Allergens
  • Ovalbumin