Characterization of Campylobacter jejuni, Campylobacter upsaliensis, and a novel Campylobacter sp. in a captive non-human primate zoological collection

J Med Primatol. 2019 Apr;48(2):114-122. doi: 10.1111/jmp.12393. Epub 2018 Dec 9.

Abstract

Background: The aim of this study was to longitudinally investigate the prevalence and characterization of Campylobacter spp. from non-human primates primate (NHP) with a history of endemic diarrhea housed at Como Park Zoo.

Methods: Fecal samples from 33 symptom-free NHP belonging to eight different species were collected weekly for 9 weeks. Species-level characterization and phylogenetic analysis of isolates included biochemical testing and 16S rRNA sequencing.

Results: Campylobacter spp. were isolated from the feces of 42% (14/33) of the primates. Three Campylobacter spp. (C upsaliensis, C jejuni, and novel Campylobacter sp.) were identified from three NHP species. A possible positive host Campylobacter species-specificity was observed. However, no statistical association was observed between the isolation of Campylobacter spp. and age and sex of the animal.

Conclusions: The study revealed the value of conducting repeated fecal sampling to establish the overall prevalence of Campylobacter in zoo-maintained NHP; it also importantly identifies a novel Campylobacter sp. isolated from white-faced saki monkeys.

Keywords: Campylobacter; captivity; diarrhea; gastrointestinal tract; non-human primate.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Zoo
  • Ape Diseases / epidemiology*
  • Ape Diseases / microbiology
  • Campylobacter / isolation & purification*
  • Campylobacter Infections / epidemiology
  • Campylobacter Infections / microbiology
  • Campylobacter Infections / veterinary*
  • Campylobacter jejuni / isolation & purification
  • Campylobacter upsaliensis / isolation & purification
  • Female
  • Haplorhini
  • Hominidae
  • Male
  • Minnesota / epidemiology
  • Monkey Diseases / epidemiology*
  • Monkey Diseases / microbiology
  • Phylogeny
  • Prevalence
  • RNA, Bacterial / analysis
  • RNA, Ribosomal, 16S / analysis
  • Species Specificity

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S