Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues

Methods Mol Biol. 2019:1893:43-51. doi: 10.1007/978-1-4939-8910-2_3.

Abstract

Drosophila melanogaster has been widely used in the study of developmental growth control and has been instrumental in the discovery and delineation of many signalling pathways that contribute to this growth, in particular the Hippo pathway. Quantitative analysis of adult tissue size has remained a vital tool in the study of tissue growth. This chapter will describe how to dissect, image, and quantify two tissues commonly used to measure growth, the Drosophila wing and eye.

Keywords: Adult eye; Adult wing; Drosophila; SEM; Tissue growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila / genetics*
  • Drosophila / growth & development*
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Eye / cytology
  • Eye / metabolism
  • Female
  • Genetic Association Studies*
  • Life Cycle Stages
  • Microscopy
  • Wings, Animal / anatomy & histology
  • Wings, Animal / metabolism

Substances

  • Drosophila Proteins