In this paper, the d/l-AP5-interfaces are firstly fabricated by attaching d-alanine-pillar[5]arene and l-alanine-pillar[5]arene (d/l-AP5) onto the gold surface, and they exhibit a significantly different chiral influence on the morphology and the adsorption quantity of the adsorbed ctDNA molecules. The research provides an ideal chiral platform for investigating chiral phenomena in biological systems.