Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization

Pei-Heng Lin; Sheng-Cih Huang; Kuang-Po Chen; Bor-Ran Li; Yaw-Kuen Li

doi:10.3390/s19010028

Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization

Sensors (Basel). 2018 Dec 21;19(1):28. doi: 10.3390/s19010028.

Authors

Pei-Heng Lin^{1

2}, Sheng-Cih Huang³, Kuang-Po Chen⁴, Bor-Ran Li^{5

6}, Yaw-Kuen Li^{7

8}

Affiliations

¹ Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].
² Department of Electrical and Computer Engineering, College of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].
³ Department of Applied Chemistry, College of Science, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].
⁴ Department of Applied Chemistry, College of Science, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].
⁵ Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].
⁶ Center for Emergent Functional Matter Science, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].
⁷ Department of Applied Chemistry, College of Science, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].
⁸ Center for Emergent Functional Matter Science, National Chiao Tung University, Hsinchu 30010, Taiwan. [email protected].

Abstract

Boronic acids (BAs) provide strong potential in orientation immobilization of antibody and the modification method is crucial for efficiency optimization. A highly effective method has been developed for rapid antibody immobilization on gold electrodes through the electrodeposition of a BA⁻containing linker in this study. Aniline-based BA forms a condense layer while antibody could automatically immobilize on the surface of the electrode. Compare to traditional self-assembled monolayer method, the electrodeposition process dramatically reduces the modification time from days to seconds. It also enhances the immobilized efficiency from 95 to 408 (ng/cm²) with a strong preference being exhibited for shorter aniline-based linkers.

Keywords: boronic acid; electrodeposition; orientated antibody immobilization; quartz crystal microbalance.

MeSH terms

Adsorption
Aniline Compounds / chemistry
Antibodies, Immobilized / chemistry*
Antibodies, Immobilized / immunology
Biosensing Techniques*
Boronic Acids / chemistry*
Electrodes
Gold / chemistry
Humans
Quartz Crystal Microbalance Techniques*

Substances

Aniline Compounds
Antibodies, Immobilized
Boronic Acids
Gold
aniline

Abstract

MeSH terms

Substances

Grants and funding