Identification and detection of USA300 methicillin-resistant Staphylococcus aureus clones with a partial deletion in the ccrB2 gene on the type IV SCCmec element

Diagn Microbiol Infect Dis. 2019 May;94(1):86-87. doi: 10.1016/j.diagmicrobio.2018.11.016. Epub 2018 Nov 24.

Abstract

Panton-Valentine leukocidin-positive highly pathogenic USA300 methicillin-resistant Staphylococcus aureus carries type IV staphylococcal cassette chromosome (SCC) mec. Here, we found USA300-like strains (named as ΨUSA300), which could not be identified as SCCmec type IV by the conventional PCR method due to a 12 bp deletion on ccrB2.

Keywords: SCCmec typing; USA300; ccrB2; ΨUSA300.

MeSH terms

  • Bacterial Toxins / genetics
  • DNA Primers / genetics
  • Diagnostic Tests, Routine / methods
  • Exotoxins / genetics
  • Genes, Bacterial*
  • Humans
  • Leukocidins / genetics
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification*
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA
  • Sequence Deletion*
  • Staphylococcal Infections / diagnosis
  • Staphylococcal Infections / microbiology
  • Virulence Factors / genetics

Substances

  • Bacterial Toxins
  • DNA Primers
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • Virulence Factors